Journal: Medicine

Article Title: Plasma miR-200c-3p, miR-100-5p, and miR-1826 serve as potential diagnostic biomarkers for knee osteoarthritis

doi: 10.1097/md.0000000000018110

Figure Lengend Snippet: Figure 3. Detections of DNMT3A, ZEB1, MMP13, and CTNNB1 protein expression in articular cartilage tissue. Target gene/internal reference control gray value comparison. A: KOA (1.36), Control (0.64); B: KOA (1.29), Control (0.63); C: KOA (1.34), Control (0.50); D: KOA(1.06), Control (0.42).

Article Snippet: These samples were used for western blotting to detect the protein levels of DNMT3A (anti-human DNMT3A antibody, #681615, R&D systems, Minneapolis, MN, USA), ZEB1 (anti-human ZEB1 antibody, #639914, R&D Systems), MMP13 (anti-human MMP13 antibody, #87512, R&D Systems), and CTNNB1 (anti-human CTNNB1 antibody, #196621, R&D Systems).

Techniques: Expressing, Control, Comparison

Journal: Medicine

Article Title: Plasma miR-200c-3p, miR-100-5p, and miR-1826 serve as potential diagnostic biomarkers for knee osteoarthritis

doi: 10.1097/md.0000000000018110

Figure Lengend Snippet: Figure 4. Correlation of miRNAs expression levels with their target mRNA levels. A is the correlation between miR-200c-3p expression level and DNMT3A mRNA level in synovial fluid; B is the correlation between miR-200c-3p expression level and ZEB1 mRNA level in synovial fluid; C is the correlation between miR-100-5p expression level and MMP13 mRNA level in synovial fluid; D is the correlation between miR-1826 expression level and CTNNB1 mRNA level in synovial fluid.

Article Snippet: These samples were used for western blotting to detect the protein levels of DNMT3A (anti-human DNMT3A antibody, #681615, R&D systems, Minneapolis, MN, USA), ZEB1 (anti-human ZEB1 antibody, #639914, R&D Systems), MMP13 (anti-human MMP13 antibody, #87512, R&D Systems), and CTNNB1 (anti-human CTNNB1 antibody, #196621, R&D Systems).

Techniques: Expressing

Journal: Alcoholism, clinical and experimental research

Article Title: Gestational choline supplementation normalized fetal alcohol-induced alterations in histone modifications, DNA methylation and POMC gene expression in ?-endorphin-producing POMC neurons of the hypothalamus

doi: 10.1111/acer.12082

Figure Lengend Snippet: Effects of gestational choline with or without ethanol on the protein level of DNA methyltransferases, MeCP2 and β-EP in cells containing β-EP in the arcuate nucleus of the hypothalamus of male PND60 offspring treated similarly as in Fig. 1. Changes in the number of β-EP neurons positive to Dnmt1 (A, B), Dnmt3a (C, D), MeCP2 (E,F) and the number of β-EP neurons (G,H) in the hypothalamus (Dnmts and MeCP2 shown in green and β-EP shown in red). Representative photographs show the double-labeled cells (A, C, E & G), indicated by arrows, in each treatment group, and histograms (B, D, F & H) show the mean ± SEM values of the percentage of β-EP cells that were double-labeled. N = 5. Dnmt1 (*p <0.05, compared to the rest). Dnmt3a, (*p<0.05, AF compared to AD and PF;a, p<0.05, PF compared to CPF). MeCP2 (*p<0.05, compared to the rest). β-EP (*p <0.05, compared to the rest;a, p<0.05, compared to AD). Data were analyzed using one-way ANOVA followed by Student Neuman-Keuls post hoc test.

Article Snippet: We have also used Dnmt1 (1:100; Santa Cruz Biotechnology; CA) and Dnmt3a (1:100; Santa Cruz) antibodies raised in goat for double staining with b-EP antibody.

Techniques: Labeling

Journal: Alcoholism, clinical and experimental research

Article Title: Gestational choline supplementation normalized fetal alcohol-induced alterations in histone modifications, DNA methylation and POMC gene expression in ?-endorphin-producing POMC neurons of the hypothalamus

doi: 10.1111/acer.12082

Figure Lengend Snippet: Effects of gestational choline with or without ethanol on the mRNA level of histone-modifying enzymes, DNA methylating enzymes and methylbinding protein MeCP2 gene in the mediobasal hypothalamus of male PND60 offspring treated similarly as in Fig. 1. Changes in mRNA levels of Set7/9 (A), G9a (B), Setdb1 (C), Dnmt1 (D), Dnmt3a (E) and MeCP2 (F). Data presented are mean ± SEM. N=5–9. Set7/9 (*p <0.05, compared to the rest). G9a (*p<0.05, compared to the rest;a, p<0.05, compared to the rest except CAF or CPF). Setdb1 (*p<0.05, compared to the rest). Dnmt1 (*p<0.05, compared to the rest). Dnmt3a (**p<0.01, compared to the rest except CPF or CAF). MeCP2 (*p<0.05, compared to the rest). Data were analyzed using one-way ANOVA followed by Student Neuman-Keuls post hoc test.

Article Snippet: We have also used Dnmt1 (1:100; Santa Cruz Biotechnology; CA) and Dnmt3a (1:100; Santa Cruz) antibodies raised in goat for double staining with b-EP antibody.

Techniques:

Journal: Alcoholism, clinical and experimental research

Article Title: Gestational choline supplementation normalized fetal alcohol-induced alterations in histone modifications, DNA methylation and POMC gene expression in ?-endorphin-producing POMC neurons of the hypothalamus

doi: 10.1111/acer.12082

Figure Lengend Snippet: Changes in protein levels of histone-modifying enzymes, DNA mcthylating enzymes and methylbinding protein MeCP2 in β-endorphin positive cells

Article Snippet: We have also used Dnmt1 (1:100; Santa Cruz Biotechnology; CA) and Dnmt3a (1:100; Santa Cruz) antibodies raised in goat for double staining with b-EP antibody.

Techniques:

Journal: The Tohoku journal of experimental medicine

Article Title: Up-regulation of DNA-methyltransferase 3A expression is associated with hypomethylation of intron 25 in human testicular germ cell tumors.

doi: 10.1620/tjem.212.177

Figure Lengend Snippet: Fig. 2. Immunological detection of DNMT3A protein in seminoma (SE), non-seminoma (NS) and adja

Article Snippet: The primary antibodies used were 200- and 400-fold dilutions of mouse monoclonal antibodies against DNMT3A (64B1446, Imgenex Co., San Diego, CA, USA) and γ - tubulin (GTU-88, Sigma-Aldrich, St. Louis, MO, USA), respectively.

Techniques:

Journal: The Tohoku journal of experimental medicine

Article Title: Up-regulation of DNA-methyltransferase 3A expression is associated with hypomethylation of intron 25 in human testicular germ cell tumors.

doi: 10.1620/tjem.212.177

Figure Lengend Snippet: Fig. 3. Analyses of DNMT3A methylation status. A: Methylation status of CpGs in the sequences

Article Snippet: The primary antibodies used were 200- and 400-fold dilutions of mouse monoclonal antibodies against DNMT3A (64B1446, Imgenex Co., San Diego, CA, USA) and γ - tubulin (GTU-88, Sigma-Aldrich, St. Louis, MO, USA), respectively.

Techniques: Methylation

Journal: Frontiers in Oral Health

Article Title: Expression of DNMTs and H3K9ac in Ameloblastoma and Ameloblastic Carcinoma

doi: 10.3389/froh.2021.751162

Figure Lengend Snippet: Multiple comparisons among groups for each protein (Kruskal–Wallis followed by Benjamini, Krieger and Yekutieli post-hoc tests).

Article Snippet: The primary antibodies incubated for 2 h were DNMT1 (ab134148, dilution 1:200, Abcam, Cambridge, UK), DNMT3A (NBP1-85961, dilution 1:100, Novus Biologicals, Newcastle, UK), DNMT3B (ab227883, dilution 1:200, Abcam) and H3K9ac (C5B11, dilution 1:500, CellSignaling, Danvers, MA, USA).

Techniques:

Journal: Frontiers in Oral Health

Article Title: Expression of DNMTs and H3K9ac in Ameloblastoma and Ameloblastic Carcinoma

doi: 10.3389/froh.2021.751162

Figure Lengend Snippet: Immunohistochemical reaction for DNMT1, DNMT3A, DNMT3B, and H3K9ac antibodies in DF (A,D,G,J) , AME (B,E,H,K) , and AC samples (C,F,I,L) , respectively. (DAB, 200×).

Article Snippet: The primary antibodies incubated for 2 h were DNMT1 (ab134148, dilution 1:200, Abcam, Cambridge, UK), DNMT3A (NBP1-85961, dilution 1:100, Novus Biologicals, Newcastle, UK), DNMT3B (ab227883, dilution 1:200, Abcam) and H3K9ac (C5B11, dilution 1:500, CellSignaling, Danvers, MA, USA).

Techniques: Immunohistochemical staining

Journal: Frontiers in Oral Health

Article Title: Expression of DNMTs and H3K9ac in Ameloblastoma and Ameloblastic Carcinoma

doi: 10.3389/froh.2021.751162

Figure Lengend Snippet: Comparison among the presence of BRAFv600e mutation and clinical and radiographic features in the AMEtma group.

Article Snippet: The primary antibodies incubated for 2 h were DNMT1 (ab134148, dilution 1:200, Abcam, Cambridge, UK), DNMT3A (NBP1-85961, dilution 1:100, Novus Biologicals, Newcastle, UK), DNMT3B (ab227883, dilution 1:200, Abcam) and H3K9ac (C5B11, dilution 1:500, CellSignaling, Danvers, MA, USA).

Techniques: Comparison, Mutagenesis